Gel Permeation Chromatography (GPC) is used for the analysis of large molecular weight polymers with organic eluents like THF and chloroform. Size Exclusion Chromatography (SEC,) also called Aqueous GPC, is for the analysis of proteins and water-soluble polymers. Columns for both have a molecular weight range and exclusion limit, and multiple columns can be used in series so small and large compounds can be separated at the same time.
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